Document Type : Original Article

Author

1 Aerospace Research Institute

2 Iran

Abstract

One of the most serious problems of the industrialized world is heavy metal contamination. As a heavy metal, lead has very harmful effects on human health even in small amounts. Therefore, removing it from water is one of the most important challenges in public health system. Microorganism application is very useful and safe in this field. Creatures living on Earth are constantly under the influence of gravity, and if it changes, they will be affected by a unique shock. Such a change has effects on the structure and function of cells by interfering with biochemical pathways and gene expression. Investigating these changes, in addition to maintaining the health of astronauts, will also be useful for improving the quality of human life on earth. In this study, the efficiency of Lactobacillus acidophilus ATCC 4356 bacteria in the bioremoval of lead from aqueous solution was investigated in microgravity and Mars gravity conditions. The results showed a decrease in lead concentration after 24-hour treatment by 82.1% under microgravity conditions, 79.6% under simulated Mars gravity conditions and 70.6% under natural Earth gravity conditions. Therefore, by reducing the gravity, it is possible to increase the efficiency of L. acidophilus in the bioremoval of lead metal

Keywords

Main Subjects

##1]            K. Arun et al., "Probiotics and gut microbiome− Prospects and challenges in remediating heavy metal toxicity," Journal of Hazardous Materials, vol. 420, p. 126676, 2021.##
##[2]          D. R. Wallace and A. B. Djordjevic, "Heavy metal and pesticide exposure: A mixture of potential toxicity and carcinogenicity," Current Opinion in Toxicology, vol. 19, pp. 72-79, 2020.##
##[3]          K. Thurmer, E. Williams, and J. Reutt-Robey, "Autocatalytic oxidation of lead crystallite surfaces," Science, vol. 297, no. 5589, pp. 2033-2035, 2002.##
##[4]          J. Oosthuizen, Environmental health: Emerging issues and practice. BoD–Books on Demand, 2012.##
##[5]          P. Arora, R. Paliwal, N. Rani, and S. Chaudhry, "Recent Trends in Bioremediation of Heavy Metals: Challenges and Perspectives," Omics Insights in Environmental Bioremediation, pp. 103-131, 2022.##
##[6]          A. Basit, S. T. Shah, I. Ullah, S. T. Muntha, and H. I. Mohamed, "Microbe-assisted phytoremediation of environmental pollutants and energy recycling in sustainable agriculture," Archives of Microbiology, pp. 1-27, 2021.##
##[7]          A. Zoghi et al., "Role of the lactobacilli in food bio-decontamination: Friends with benefits," Enzyme and Microbial Technology, vol. 150, p. 109861, 2021.##
##[8]          R. M. Abdel-Megeed, "Probiotics: a promising generation of heavy metal detoxification," Biological trace element research, vol. 199, no. 6, pp. 2406-2413, 2021.##
##[9]          R. Massoud, K. Khosravi‐Darani, A. Sharifan, G. Asadi, and A. Zoghi, "Lead and cadmium biosorption from milk by Lactobacillus acidophilus ATCC 4356," Food Science & Nutrition, vol. 8, no. 10, pp. 5284-5291, 2020.##
##[10]       Z. Afsharian, M. Salavatifar, and K. Khosravi_Darani, "Impact of simulated microgravity on bioremoval of heavy-metals by Lactobacillus acidophilus ATCC 4356 from water," Heliyon, vol. 8, no. 12, p. e12307, 2022.##
##[11]       A. Zoghi et al., "Effect of pretreatments on bioremoval of metals and subsequent exposure to simulated gastrointestinal conditions," Quality Assurance and Safety of Crops & Foods, vol. 14, no. 3, pp. 145-155, 2022.##
##[12]       G. Senatore, F. Mastroleo, N. Leys, and G. Mauriello, "Effect of microgravity & space radiation on microbes," Future Microbiology, vol. 13, no. 07, pp. 831-847, 2018.##
##[13]       B. Huang, D.-G. Li, Y. Huang, and C.-T. Liu, "Effects of spaceflight and simulated microgravity on microbial growth and secondary metabolism," Military Medical Research, vol. 5, no. 1, pp. 1-14, 2018.##
##[14]       M. Salavatifar, N. Mosallaei, and A. H. Salmanian, "Heterologous Expression of Shiga-Like Toxin Type 2 in Microgravity Condition," Journal of Space Science and Technology, 2022.##
##[15]       S. Turroni et al., "Temporal dynamics of the gut microbiota in people sharing a confined environment, a 520-day ground-based space simulation, MARS500," Microbiome, vol. 5, pp. 1-11, 2017.##
##[16]       M. Salavatifar, S. M. Ahmadi, S. D. Todorov, K. Khosravi-Darani, and A. Tripathy, "Impact of microgravity on virulence, antibiotic resistance, and gene expression in beneficial and pathogenic microorganisms," Mini Reviews in Medicinal Chemistry, 2023.##
##[17]       L. Yuan et al., "Long-term simulated microgravity alters gut microbiota and metabolome in mice," Frontiers in Microbiology, vol. 14, 2023.##
##[18]       D. Pearce, C. Moissl-Eichinger, T. Kuehnast, C. Abbott, and A. Mahnert, "The crewed journey to Mars and its implications for the human microbiome," 44th COSPAR Scientific Assembly. Held 16-24 July, vol. 44, p. 3278, 2022.##
##[19]       S. Sieuwerts, F. A. De Bok, E. Mols, W. M. De Vos, and J. van Hylckama Vlieg, "A simple and fast method for determining colony forming units," Letters in applied microbiology, vol. 47, no. 4, pp. 275-278, 2008.##
##[20]       M. R. Hadiani, K. Khosravi-Darani, N. Rahimifard, and H. Younesi, "Assessment of mercury biosorption by Saccharomyces cerevisiae: response surface methodology for optimization of low Hg (II) concentrations," Journal of environmental chemical engineering, vol. 6, no. 4, pp. 4980-4987, 2018.##
##[21]       M. R. Hadiani, K. K. Darani, N. Rahimifard, and H. Younesi, "Biosorption of low concentration levels of Lead (II) and Cadmium (II) from aqueous solution by Saccharomyces cerevisiae: Response surface methodology," Biocatalysis and agricultural biotechnology, vol. 15, pp. 25-34, 2018.##
##[22]       K. H. Hasenstein, J. Van Loon, and D. Beysens, "Clinostats and other rotating systems—design, function, and limitations," Generation and applications of extra-terrestrial environments on earth, vol. 14, pp. 147-156, 2015.##
##[23]       Z. Hajebrahimi, "3-D clinostat for microgravity simulation in cellular and molecular studies," Journal of Technology in Aerospace Engineering, vol. 1, no. 2, pp. 27-33, 2017.##
##[24]       P. Gupta and B. Diwan, "Bacterial exopolysaccharide mediated heavy metal removal: a review on biosynthesis, mechanism and remediation strategies," Biotechnology Reports, vol. 13, pp. 58-71, 2017.##
##[25]       L. Mauclaire and M. Egli, "Effect of simulated microgravity on growth and production of exopolymeric substances of Micrococcus luteus space and earth isolates," FEMS Immunology & Medical Microbiology, vol. 59, no. 3, pp. 350-356, 2010.##
##[26]       Z. Chen, X. Pan, H. Chen, X. Guan, and Z. Lin, "Biomineralization of Pb (II) into Pb-hydroxyapatite induced by Bacillus cereus 12-2 isolated from Lead–Zinc mine tailings," Journal of hazardous materials, vol. 301, pp. 531-537, 2016.##
##[27]       S. Xing et al., "Lead biosorption of probiotic bacteria: effects of the intestinal content from laying hens," Environmental Science and Pollution Research, vol. 24, no. 15, pp. 13528-13535, 2017.##
##[28]       C. A. Nickerson, C. M. Ott, J. W. Wilson, R. Ramamurthy, and D. L. Pierson, "Microbial responses to microgravity and other low-shear environments," Microbiology and Molecular Biology Reviews, vol. 68, no. 2, pp. 345-361, 2004.##